| Tuesday, May 16 |
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| 9:00-9:40 |
Ales Benda |
BioCev |
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| 9:40-10:20 |
Wolfgang Becker |
Becker & Hickl GmbH, Berlin |
Fluorescence lifetime imaging by multi-dimensional TCSPC – basics, advanced techniques, applications |
| Coffee Break |
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| 10:40-11:20 |
Klaus Suhling |
King’s College London |
Wide-field TCSPC-based FLIM |
| 11:20-12:00 |
Thomas Blacker |
University College London |
Photophysics of NADH and NADPH |
| Lunch Break |
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| 1:20-2:00 |
Angelika Rück |
University Ulm, Germany |
FLIM and PLIM for cell metabolism and oxygen sensing with applications in neurology and PDT |
| 2:00-2:40 |
Ruslan Dmitriev |
University College Cork, Ireland |
Multi-parametric FLIM-PLIM of 3D tumour and stem cell-based models: measuring O2 together with temperature and cell cycle |
| Coffee Break |
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| 3:00-3:40 |
Martin Hammer |
University Jena, Germany |
Fluorescence lifetime imaging at the retina in a clinical setting |
| 3:40-4:20 |
Volker Huck |
University Medical Center Hamburg-Eppendorf, Germany |
Multiphoton fluorescence lifetime imaging at patient´s bedside – of morphometric analysis and medical applications |
| 4:20-4:40 |
Jana Humpolíčková |
Institute of Organic Chemistry and Biochemistry of the CAS, Prague |
A FLIM-FRET Approach for the Determination of Membrane Protein Oligomerization in Live Cell Membranes: Case Study of Rhomboid-Related Protein 2 (RHBDL2) |
| 4:40-5:00 |
Jakub Rohlena |
IBT Prague |
Using NAD(P)H autofluorescence to asses the activity of the mitochondrial electron transport chain in live cells |
| 5:00- |
Demos |
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| Wednesday, May 17 |
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| 9:00-9:40 |
Martin Hof |
Inst. Phys. Chem. Prague, Czech Republic |
Use of fluorescence spectroscopy in synthetic biology |
| 9:40-10:20 |
Michael Börsch |
University Jena, Germany |
Monitoring conformational dynamics of the rotary subunit F in the A 1-complex of archaeal ATP synthase by single-molecule FRET |
| Coffe Break |
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| 10:40-11:20 |
Dusan Chorvat |
International Laser Center Bratislava, Slovakia |
Multi-modal optical imaging and spectroscopy of intrinsic fluorophores |
| 11:20-12:00 |
Patrick Schäfer |
University Ulm, Germany, Clinics of Neurology |
NADH FLIM in neuroenergetics |
| Lunch Break |
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| 1:20-2:00 |
Alexandra Meleshina |
Nizhnii Novgorod Medical State Academy, Russia |
Complex analysis of metabolic status, intracellular pH, viscosity and cytoskeleton of human mesenchymal stem cells during osteogenic and chondrogenic differentiation by fluorescent microscopy and FLIM |
| 2:00-2.40 |
Elena Koshel |
Saint Petersburg State University |
The examination of new phosphorescent probes based on transition metals complexes for Phosphorescent Lifetime Imaging Microscopy (PLIM) |
| Coffe Break |
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| 3:00-3:40 |
Anca Margineanu |
Max Delbrueck Centre for Molecular Medicine, Berlin, Germany |
Quantitative molecular imaging across scales using fluorescence lifetime imaging (FLIM) |
| 3:40-4:00 |
Goutam Pramanik |
Institute of Organic Chemistry and Biochemistry of the CAS, Prague |
Near InfraRed (NIR) Emitting Gold Nanoclusters for Bioimaging Applications |
| 4:00-4:20 |
Hana Uhlířová |
CEITEC |
Regulation of tissue metabolism by variation in O2 availability: 2-photon imaging of NADH in cerebral cortex in vivo |
| 4:20-5:00 |
Wolfgang Becker |
Becker & Hickl GmbH |
Fast FLIM at an Image rate of 5 fps |
| 5:00- |
Demos |
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