Agenda
Tuesday, May 16 | |||
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9:00-9:40 | Ales Benda | BioCev | |
9:40-10:20 | Wolfgang Becker | Becker & Hickl GmbH, Berlin | Fluorescence lifetime imaging by multi-dimensional TCSPC – basics, advanced techniques, applications |
Coffee Break | |||
10:40-11:20 | Klaus Suhling | King’s College London | Wide-field TCSPC-based FLIM |
11:20-12:00 | Thomas Blacker | University College London | Photophysics of NADH and NADPH |
Lunch Break | |||
1:20-2:00 | Angelika Rück | University Ulm, Germany | FLIM and PLIM for cell metabolism and oxygen sensing with applications in neurology and PDT |
2:00-2:40 | Ruslan Dmitriev | University College Cork, Ireland | Multi-parametric FLIM-PLIM of 3D tumour and stem cell-based models: measuring O2 together with temperature and cell cycle |
Coffee Break | |||
3:00-3:40 | Martin Hammer | University Jena, Germany | Fluorescence lifetime imaging at the retina in a clinical setting |
3:40-4:20 | Volker Huck | University Medical Center Hamburg-Eppendorf, Germany | Multiphoton fluorescence lifetime imaging at patient´s bedside – of morphometric analysis and medical applications |
4:20-4:40 | Jana Humpolíčková | Institute of Organic Chemistry and Biochemistry of the CAS, Prague | A FLIM-FRET Approach for the Determination of Membrane Protein Oligomerization in Live Cell Membranes: Case Study of Rhomboid-Related Protein 2 (RHBDL2) |
4:40-5:00 | Jakub Rohlena | IBT Prague | Using NAD(P)H autofluorescence to asses the activity of the mitochondrial electron transport chain in live cells |
5:00- | Demos | ||
Wednesday, May 17 | |||
9:00-9:40 | Martin Hof | Inst. Phys. Chem. Prague, Czech Republic | Use of fluorescence spectroscopy in synthetic biology |
9:40-10:20 | Michael Börsch | University Jena, Germany | Monitoring conformational dynamics of the rotary subunit F in the A 1-complex of archaeal ATP synthase by single-molecule FRET |
Coffe Break | |||
10:40-11:20 | Dusan Chorvat | International Laser Center Bratislava, Slovakia | Multi-modal optical imaging and spectroscopy of intrinsic fluorophores |
11:20-12:00 | Patrick Schäfer | University Ulm, Germany, Clinics of Neurology | NADH FLIM in neuroenergetics |
Lunch Break | |||
1:20-2:00 | Alexandra Meleshina | Nizhnii Novgorod Medical State Academy, Russia | Complex analysis of metabolic status, intracellular pH, viscosity and cytoskeleton of human mesenchymal stem cells during osteogenic and chondrogenic differentiation by fluorescent microscopy and FLIM |
2:00-2.40 | Elena Koshel | Saint Petersburg State University | The examination of new phosphorescent probes based on transition metals complexes for Phosphorescent Lifetime Imaging Microscopy (PLIM) |
Coffe Break | |||
3:00-3:40 | Anca Margineanu | Max Delbrueck Centre for Molecular Medicine, Berlin, Germany | Quantitative molecular imaging across scales using fluorescence lifetime imaging (FLIM) |
3:40-4:00 | Goutam Pramanik | Institute of Organic Chemistry and Biochemistry of the CAS, Prague | Near InfraRed (NIR) Emitting Gold Nanoclusters for Bioimaging Applications |
4:00-4:20 | Hana Uhlířová | CEITEC | Regulation of tissue metabolism by variation in O2 availability: 2-photon imaging of NADH in cerebral cortex in vivo |
4:20-5:00 | Wolfgang Becker | Becker & Hickl GmbH | Fast FLIM at an Image rate of 5 fps |
5:00- | Demos |